Interpreting qPCR & MST results involves analyzing Ct values and considering the experiment's context. Here's a general guideline:
- Low Ct Values (e.g., Ct < 29): Indicate high target abundance.
- Moderate Ct Values (e.g., 30 < Ct < 35): Suggest moderate target abundance.
- High Ct Values (e.g., Ct > 35): Indicate low target abundance, with values close to 40 approaching the assay's detection limit.