Why Test for micC?
Many industries already perform microbial testing to assess overall microbial presence, but not all microbes cause corrosion. The micC qPCR assay is specifically designed to detect SRB involved in direct corrosion mechanisms, providing a more targeted approach to MIC monitoring. Unlike general microbial tests, micC identifies microbes that can actively contribute to metal deterioration.
Integrating micC into a microbial monitoring program can:
- Detect MIC threat early before visible damage occurs.
- Differentiate between general microbial activity and corrosion-causing bacteria.
- Improve maintenance planning by identifying high-risk environments or sampling locations.
- Reduce unplanned shutdowns and asset failures.
Technical Considerations for micC Testing
Dual-Assay Design and Result Interpretation
The micC assay differs from standard qPCR tests in that it uses two separate reactions to target three variations of the micC gene. This enhances coverage and reduces the likelihood of false positives or negatives. However, it also requires more processing steps and cumulative result interpretation.
- Each sample must be tested in two wells, and can be combine to determine the total micC concentration.
- Example: If micC1 = 100 cells/mL and micC 2/3 = 100 cells/mL, the total micC result is 200 cells/mL.
This approach provides a more accurate representation of the presence and abundance of corrosive SRB.
Sample Handling Requirements
Due to the dual-assay format, proper loading and sample preparation are critical. Each sample requires two wells per analysis, which is different from other single-well assays.
- For detailed workflow instructions, refer to the article "How to set up micC qPCR assay?" or the Assay Kit Test Kit Instructions
Software Update Required
The micC assay is supported by an updated version of the qPCR analysis software. Without this update, results may not be interpreted correctly.
- Ensure your system is running on the latest software GeneCount Device Software Downloads
Need Help of Want to Learn More?
Our team of experts is here to assist you with your microbiological monitoring needs. Whether you have questions, need technical support, or want to learn more about GeneCount qPCR testing, contact LuminUltra® Support to get started.