DNA purification and qPCR assay setup require good pipettor techniques to ensure accurate liquid volumes are used throughout the protocol to get the best results. Learn more about Best practices for qPCR pipetting in this article.
Micropipettor anatomy
This image illustrates the key components of a micropipettor, including the plunger, ejector, volume dial, volume display, ejector slide, and pipette tip. It also features a table demonstrating the pipette model range, dial reading, and actual volume. Additionally, it provides a helpful tip for using periods to indicate decimals or the thousands place in pipetting, following industry standards.
Step-by-step instructions
Step One: Press firmly downwards to attach the pipette tip.
Pro Tip: Avoid touching the tip on any surface to reduce contamination
Step Two: Place the micropipette tip into liquid, then push the plunger to first stop and slowly release.
Step Three: Position your micropipette tip over your PCR tube, push the plunger to first stop and continue pushing to the second stop.
Step Four: Dispose of pipette tip by ejecting it into biohazard waste
Tips for success
- Label your PCR tubes on the side instead of the top
- Ensure that all your tubes have a consistent liquid level. Inconsistent liquid levels indicate improper pipetting technique.
- Ensure all your liquid is collected at the bottom of your PCR tubes and not on the sides and cap.