How to set up micC qPCR assay?

Overview

The micC qPCR assay is designed to detect corrosive sulfate-reducing bacteria (SRB) that contributes directly to microbially influenced corrosion (MIC). Unlike standard qPCR assays that usually test for only one genetic target per reaction, this assay includes three gene targets spread across two different reaction tubes.

• Tube 1: Tests for micC-1
• Tube 2: Tests for micC-2 and micC-3, plus an internal control

To rest a single sample, you will need to run both tubes, one for micC-1, and one for micC-2/3.

micC Plate Layout Guide

Below is a simple layout to help you organize your samples.

Each row represents one type of micC assay

• Blue wells (Row A) = micC-1
• Green wells (Row B) = micC-2/3

Each column is one sample. For example:

• A1 = Sample 1 (micC-1)
• B1 = Sample 1 (micC-2/3)

Note: Keep assay types clearly labeled to avoid confusion. Use a fine-point permanent marker to write on tube caps or PCR plate lids if needed.

Project Files and Assay List Update Required

The micC assay requires an updated version of the GeneCount Voyager Project Files and GeneCount qKit Wizard Assay for proper experimentation setup, and result interpretation.

Updating the GeneCount Voyager Project Files

1. Connect the voyager to your WiFi network.
2. Launch GeneCount Voyager Software.
3. Project Files should automatically update when launching the software.
4. To confirm projects are updated, select the projects tab from the Voyager software.
5. Press load projects web. 

Updating GeneCount qKit Report Wizard

1. Connect the Voyager to your WiFi network.
2. Launch GeneCount qKit Report Wizard.
3. You will be prompted to download the latest version, select "Yes".
4. After Installation, confirm that your qPCR instrument can distinguish between micC-1 and micC-2/3 targets.

Note: Without this update, results may not be properly interpreted or recorded.

Interpreting Results

The result per sample is the combined total of micC-1 and micC-2/3 concentrations. This total indicates the presence and relative abundance of corrosive SRB in your system.

Example:

• micC-1 result = 11.9 cells/mL
• micC-2/3 result = 9.22 cells/mL
• Total micC result = 21.1 cells/mL

Need Help or Want to Learn More?

Our team of experts is here to assist you with your microbiological monitoring needs. Whether you have questions, need technical support, or want to learn more about GeneCount qPCR testing, contact LuminUltra® Support to get started.