Control |
Negative Control |
Positive Control |
Internal Control |
Definition |
The negative control consists of a qPCR master mix and nuclease-free water. This should not amplify. | The positive control mixes the highly concentrated target of interest and nuclease-free water. This should amplify. | LuminUltra offers several probe-based assays which contain internal controls. This should amplify. |
Use |
The negative control ensures no template control omits any DNA or RNA template from a reaction and serves as a general control against contamination. | The best practice for each target assay is to include a positive control. The positive control serves as a control to determine whether or not the qPCR reaction conditions are optimal. | Internal Controls are intended to confirm that no inhibitors are present in the reaction and that the master mix amplifies properly to validate a qPCR result. Failed internal controls may indicate the presence of inhibitors. |
Failed Control in qPCR Run
If a control in your qPCR run has failed, it is important to assess the impact on your results before accepting them. Please read the following article: A control failed in my run, can I accept my results?