Proper handling and storage of reagents are essential to ensure accurate and reliable ATP testing results. Following these guidelines will help maintain reagent integrity and extend their shelf life.
General Guidelines
- Store all reagents at a stable temperature between 4–25°C (39–77°F).
- Avoid direct sunlight, excessive heat, or freezing, as temperature fluctuations can degrade reagent quality.
- Inspect reagents for signs of degradation (e.g., discoloration, sedimentation, or unusual odor) before each use.
- Always use reagents before their labeled expiration date to ensure accurate results.
Specific Storage Tips
Reagent |
Storage & Shelf Life |
Other Considerations |
| Reagent Z + Buffer |
Recommended Storage: 4 to 25°C / 39 to 77°F Shelf Life: 2 years |
Reagent Z should be stored at 4–25°C (39–77°F) until it is rehydrated. After rehydration, it must be refrigerated between uses to maintain its activity. Properly stored, rehydrated Reagent Z remains effective for up to 3 months, but it is important to periodically test its performance to confirm accuracy. |
| Reagent X |
Recommended Storage: 4 to 25°C / 39 to 77°F Shelf Life: 2 years |
To maintain the integrity of Reagent X, ensure the container remains tightly sealed at all times. Exposure to air or contaminants can degrade its effectiveness, so careful handling is essential to preserve its stability. When stored properly and unopened, these reagents have a shelf life of up to 2 years. |
| Standard |
Recommended Storage: 4 to 25°C / 39 to 77°F Shelf Life: 2 years |
Once opened, Standard must be used within 3 months to guarantee accurate calibration results. To prevent degradation, avoid leaving the bottle uncapped for extended periods, as excessive exposure to air, heat, or light can reduce its reliability. Always store Standard between 4–25°C (39–77°F) and discard it after the three-month period. When stored properly and unopened, these reagents have a shelf life of up to 2 years. |
| Rinse |
Recommended Storage: 4 to 25°C / 39 to 77°F Shelf Life: 2 years |
Rinse Reagent should be used generously during testing to effectively remove any residues from filters and surfaces. Proper rinsing ensures accurate ATP measurements and prevents contamination from interfering with subsequent test steps. When stored properly and unopened, these reagents have a shelf life of up to 2 years. |
| Soak |
Recommended Storage: 4 to 25°C / 39 to 77°F Shelf Life: 2 years |
Soak Reagent stabilizes free-floating ATP and helps ensure accurate measurements. It must be stored at 4–25°C and never frozen, as freezing can interfere with its effectiveness. Proper storage guarantees reliable performance throughout its lifespan. Soak Reagent stabilizes free-floating ATP and helps ensure accurate measurements. It must be stored at 4–25°C and never frozen, as freezing can interfere with its effectiveness. Proper storage guarantees reliable performance throughout its lifespan. |
| Reagent D |
Recommended Storage: 4 to 25°C / 39 to 77°F Shelf Life: 2 years |
When stored properly and unopened, these reagents have a shelf life of up to 2 years.
|
Best Practices for Reagent Handling
To avoid contamination, always keep reagents in their original containers with lids tightly sealed when not in use. Never interchange caps between reagent bottles, as this can introduce unwanted contaminants and compromise test results. Discard expired reagents promptly to prevent inaccuracies, and always handle reagents with clean gloves to maintain their purity.
Why Proper Storage Matters
Reagent performance directly impacts the accuracy and reliability of ATP test results. Improper storage conditions can lead to reduced sensitivity, false readings, and inconsistent data, which may undermine your microbial monitoring efforts. By adhering to these guidelines, you can maintain reagent quality and ensure dependable, high-quality results every time.
Need Help or Want to Learn More?
Our team of experts is here to assist you with your microbiological monitoring needs. Whether you have questions, need technical support, or want to learn more about LifeCheckTM ATP testing, contact LuminUltra® Support to get started.